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KMID : 0378019910340020089
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New Medical Journal
1991 Volume.34 No. 2 p.89 ~ p.92
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Differentiation of Proteinuria Using SDS -PAGE
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Abstract
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Proteinuria is one of markers of renal disease. The detection of a proteinuria should be occasion for further medical examination. Usually, a screening for proteinuria is performed by means of urine dipsticks. This method is very important for a preselection of. potential patients with kidney disease, but it has some disadvantages. In fact, some renal diseases are connected with a form of proteinuria which cannot be detected with the help of urine test strips.
A modern laboratory diagnosis of proteinuria should allow not only the detection of any pathological protein excretion but also the quantitation and differentiation of protein patterns. Different forms of a proteinuria (glomerular proteinuria and tubular prteinuria) can be defined using sodium dodecylsulfate polyacrylamide gel elect rophoresis (SDS -PAGE).
We analyzed 30 patients proteinuria using SDS-PAGE. The SDS-PAGE has been shown to be useful for molecular size separation of urinary proteins. A routine method of SDSPAGE we used is described.
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